BoxA 2D (negative control of BoxA wt), LPS-free
BoxA 2D is the mutant form of BoxA (DNA binding domains of HMGB1 protein) in which the two cysteines are replaced with aspartic acid.
It consists of 89 amino acids and has a calculated molecular mass of approximately 10.4 kDa.
BoxA 2D in migration assay cannot block the HMGB1 induced migration of fibroblasts.
It contains only trace amounts of LPS (<0.4 ng/mg protein).
BoxA 2D variant can be used as negative control of BoxA wt.
It has the sequence:
[“MGKGDPKKPR GKMSSYAFFV QTDREEHKKK HPDASVNFSE FSKKDSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKF” ]
Molecular Mass: BoxA 2D from HMGB1 consists of 89 amino acid and has a calculated molecular mass of approximately 10.4 kDa.
Purity: The purified protein is >98% homogeneous (electrophoresis and mass spectrometry).
Endotoxin Level: The purified protein is free from LPS (Cambrex Limulus Amoebocyte Assay QCL-1000, <0.4 ng LPS per mg protein).
Buffer: 50 mM HEPES-Na pH 7.9, 500 mM NaCl, 0.5 mM DTT.
Storage: the protein is shipped lyophilized. Once resuspended can be stored frozen at -20°C.
This product is intended for research only, and cannot be used on humans.
Complete Name: BoxA 2D from High Mobility Group 1
Other Names: A-Box HMGB1 mutant, Box-A from HMGB1 mutant, BoxA from High Mobility Group 1 mutant, HMGB1 aspartic acid mutant, 2D mutant