anti HMGB1 monoclonal antibody
The mouse monoclonal IgG1 DPH1.1 was generated by injecting C57BL/6 mice with the 17-mer peptide P1 (KGKPDAAKKGVVKAEKS). Hybridomas were generated from splenocytes by standard techniques and tested by ELISA against the immunogen and full-length HMGB1.
DPH1.1 mAb recognizes all mammalian HMGB1s, including human, mouse and rat. Does not recognize HMGB2.
DPH1.1 mAb can be used for Western blot (WB) and immunohistochemistry (IHC).
DPH1.1 mAb blocks HMGB1-elicited cell migration in trans-well migration assays.
In vivo, DPH1.1 mAb administered intravenously (220 μg/mouse) blocks recruitment of inflammatory cells to sites of necrosis and infection.
This product is intended for research only, and cannot be used on humans.
Publications where this product was used:
Sitia G, Iannacone M, Aiolfi R, Isogawa M, van Rooijen N, Scozzesi C, Bianchi ME, von Andrian UH, Chisari FV and Guidotti LG (2011) Kupffer cells hasten resolution of liver immunopathology in mouse models of viral hepatitis. PLoS Pathog 7: e1002061