BoxAB from HMGB1 (Tailless-HMGB1, 20 kDa), human LPS-Free
BoxAB (Tailless-HMGB1) is a recombinant fragment of HMGB1 including both DNA-binding domains, BoxA and BoxB, lacking the acidic C-terminal tail.
BoxAB from HMGB1 includes both BoxA and BoxB domains connected as in the natural sequence but lacks the negatively charged C-terminal tail. This tailless form retains the structural integrity and receptor-binding capacity of full-length HMGB1, while showing altered functional behavior in redox-dependent signaling and immune activation.
BoxAB is ideal for studying the mechanisms of HMGB1-mediated inflammation, receptor binding (RAGE, TLR2, TLR4), and DNA interaction without interference from the acidic tail. The protein is fully compatible with in vitro biochemical assays, cell culture studies, and receptor-ligand binding analyses.
The product is produced in E.coli from an expression plasmid coding for the human HMGB1 sequence (amino acids 1–176) without any tags or modifications, ensuring natural folding and charge distribution. The protein has the following sequence:
MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVV
Molecular Mass: BoxAB from HMGB1 consists of 176 amino acids with a calculated molecular mass of approximately 20.0 kDa.
Purity: >95% homogeneous by SDS-PAGE. Contains no nucleic acids.
Endotoxin Level: LPS-free (Pierce™ Chromogenic Endotoxin Quant Kit, <0.1 EU/mL). Contains <0.006% v/v of Triton X-114 due to LPS removal; remaining traces can be removed upon request.
Buffer & Reconstitution: lyophilized protein should be reconstituted in distilled water, resulting in a solution containing 50 mM HEPES pH 7.9, 500 mM NaCl, 0.5 mM DTT.
Storage: shipped lyophilized. Once resuspended, store frozen at -20°C.
This product is intended for research use only and cannot be used on humans.
References:
- Wang et al. DNA-mediated proteolysis by neutrophil elastase enhances binding activities of the HMGB1 protein. J Biol Chem. 2022, 298(11):102577-7.
- Mantonico et al. The acidic intrinsically disordered region of the inflammatory mediator HMGB1 mediates fuzzy interactions with CXCL12. Nat Commun. 2024, 30: 1201-38
- Müller et al. Thermodynamics of HMGB1 interaction with duplex DNA. Biochemistry 2001, 40:10254–61
- Yang et al. Reversing established sepsis with antagonists of endogenous high-mobility group box 1. PNAS 2004, 101:296–301.
- Sitia et al. Treatment with HMGB1 inhibitors diminishes CTL-induced liver disease in HBV transgenic mice. J Leukoc Biol 2007, 81:100–7.
- Kou et al. HMGB1 cleavage by complement C1s and its potent anti-inflammatory product. Front Immunol 2023, 14:1151731.
Complete Name: BoxAB (Tailless) from High Mobility Group Box 1
Other Names: HMGB1 Tailless, BoxA+BoxB HMGB1, HMGB1(1–176)
