HMGB1 is produced in E.coli from an expression plasmid coding for the human protein (Müller et al. Thermodynamics of HMGB1 interaction with duplex DNA. Biochemistry 2001, 40: 10254-61):
It has the sequence:
“MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEDD EEDEEDEEEE EEEEDEDEEE DDDDE” ]
Molecular Mass: HMGB1 consists of 215 amino acid residues and has a calculated molecular mass of approximately 24,8 kDa. It migrates at a position of approximately 30 kD in SDS-PAGE gels, possibly because of the unusual number of positively charged amino acids it contains.
Purity: The purified protein is >95% homogeneous (electrophoresis and mass spectrometry).
Activity: This preparation has low cytokine activity, but is fully active as a DNA binding protein or as an antigen.
Buffer: 50 mM HEPES buffer pH 7.9, 500 mM NaCl, 0.5 mM DTT.
Storage: We advise to keep the product frozen at -20°C.
This product is intended for research only, and cannot be used on humans.
Publications where this product was used:
Mollica L et al. Glycyrrhizin binds to High Mobility Group Box 1 protein (HMGB1) and inhibits its cytokine activities. Chem Biol 2007, 14: 431-41
Complete Name: High Mobility Group 1
Other Names: Amphoterin-1, High mobility group protein 1,HMG-1, SBP-1, high mobility group protein B1, high-mobility group box 1, Amphoterin, HMG1, HMGB1, SBP-1, recombinant HMGB1